The method Clustal uses to construct the alignment is called pairwise progressive sequence alignment. This heuristic method first does a pairwise sequences alignment for all the sequence pairs that can be constructed from the sequence set. A dendrogram guide tree of the sequences is then done according to the pairwise similarity of the sequences.
Finally a multiple sequence alignment is constructed by aligning sequences in the order, defined by the guide tree. From the Clustal X window you can use the pull-down menus to import sequences, align sequences, save sequences or alignments, and perform other program tasks. Download Clustal W2 clustalx-windows-xp Download Clustal W2 clustalx-mac-universal Clustal 2. Online versions which might be out of date can be found here: Clustal W and Clustal X. A very nice tutorial which also covers advanced features like using secondary structures for profile alignment is "Using Clustal X for multiple sequence" by Jarno Tuimala local mirror.
You can reach us at. If you want to report a bug please make sure, that you are using the most recent version of Clustal. Please also check if the bug has already been reported on our Bugzilla webpage. We would like to thank all Clustal users who have send in feedback, bug reports and feature requests. Recent work on Clustal was mainly funded by Science Foundation Ireland. Webservers You don't necessarily have to go through the hassle to install Clustal on your computer. Clustal W and Clustal X version 2.
Bioinformatics , 23, Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res. Multiple sequence alignment with Clustal X. Trends Biochem Sci. Methods Enzymol.
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